Nanotools Lc3 PDF
microtubule-associated protein1 light chain 3
www.nanotools.de SUMMARY To date, LC3, a mammalian homolog of yeast Atg8, is the only reliable marker of autophagosomes. Tracking the conversion of LC3-I to LC3-II is indicative of autophagic activity. As half-life of LC3-II is short because autophagosomes ...
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Mab LC3-5F10 specifically recognizes both forms of endogenous LC3, the cytoplasmic LC3-I (18 kDa) as well ... e.g. nanoTools product #3031-500/CPPT or #3031-3000/CPPT. IgG1 Isotype synthetic peptide conjugated to hemocyanin Immunogen N-terminus of LC3-B Epitope
p62/SQSTM1BindsDirectlytoAtg8/LC3toFacilitate ...
polyclonal antibody (Rockland Immunochemicals); anti-LC3 monoclonal antibody (NanoTools Antikorpertechnik); anti-actin and anti-LAMP1 monoclonal antibodies (Sigma); and horseradish peroxidase-conjugated anti-mouse and anti-
NCL Method GTA-11 - Home - Nanotechnology Characterization Lab
NCL Method GTA-11 . Autophagic Dysfunction Assay: Qualitative Analysis of MAP LC3-I to LC3-II Conversion . by Western Blot . Nanotechnology Characterization Laboratory
Supplemental Figure Legends Figure S1. Autophagy is activated ...
The following antibodies were used for immunohistochemistry: LC3 (NanoTools), Dec1 (Giatromanolaki et al. 2003) and Ki67 (Neomarkers). The following antibodies were used for immunofluorescence; ATG12 (Cell Signaling) and HA (clone 12CA5; Cancer Research UK in house).
OriginalArticle PossibleRoleforExternalEnvironmentalStimuliin ...
from Nanotools) with three anti-LC3 antibodies (0231s0104 from Nanotools, PD014 from MBL and PM036 from MBL) indicatedthatPD014andPM036labeledLC3-Iand-IIbands but 0231s0104 did not. In theordinary formalin-fixed and paraffin-embedded sections of human lymph node with ne-
FoxO Transcription Factors Promote Autophagy in Cardiomyocytes
Signaling)(1:700);LC3(NanoTools)(0.5 g/ml);AKT(Cell Signaling) (1:2000); phospho-AKT (Ser-473) (1:1000); Sirt1 (Millipore) (1:1000). GAPDH (Santa Cruz Biotechnology) (1:10,000) antibody was used as a loading control. Statistical
Apoptosis-Inducing Factor Regulates Skeletal Muscle ...
1/100) from NanoTools; LC3 (polyclonal, 1/100) from MBL; slow type I MyHC (NCL-MHCS, monoclonal, 1/20), all fast type II ... For LC3 staining, muscle sections were fixed 20 minutes at 4uC in 4% paraformaldehyde and immersed in blocking solution
ULK-Atg13-FIP200 Complexes Mediate mTOR Signaling to the ...
transferred to PVDF membranes and probed with anti-LC3 mouse polyclonal antibody (Nanotools) and anti-p62 antibody (Bethyl Laboratories). In Vivo Labeling 293T cells on 6-cm plates were transduced with plasmids and washed with
p62/SQSTM1 Binds Directly to Atg8/LC3 to Facilitate ...
p62/SQSTM1 Binds Directly to Atg8/LC3 to Facilitate Degradation of Ubiquitinated Protein Aggregates by Autophagy Serhiy Pankiv1, Terje Høyvarde Clausen1, Trond Lamark1, Andreas Brech2, Jack-Ansgar Bruun1,
Glucocorticoid dose determines osteocyte cell fate
antibody for the autophagy marker, LC3 (LC3-5F10; 30 g/mouse; NanoTools, San Diego, CA, USA) was injected into 3 mice in each of the 4 experimental groups to label the autophagic osteocytes. All animals were treated accord-
Autophagy contributes to therapy-induced degradation of the ...
1:1000), mouse anti-LC3 (Nanotools; 1:100), mouse anti-PML (Santa Cruz ... creased LC3-II levels), and inhibition of p70S6K phosporylation (Figure 5B) but had no effect on cell survival (supplemental Figure 5B). Taken together, our results show that PML/RARA clear-
Autophagy contributes to therapy-induced degradation of the ...
1:1000), mouse anti-LC3 (Nanotools; 1:100), mouse anti-PML (Santa Cruz ... creased LC3-II levels), and inhibition of p70S6K phosporylation (Figure 5B) but had no effect on cell survival (supplemental Figure 5B). Taken together, our results show that PML/RARA clear-
AutophagyInductionandAutophagosomeClearancein Neurons ...
(1:1000; TUJ-1; R&D Systems), overnight for LC3 (1:250; Nanotools) and cathepsin D (1:5000; in house) at 4°C. Cells were washed (three times) in PBS for 10 min before incubation with TRITC (tetramethyl-rhodamine isothiocyanate)- and FITC (fluorescein isothiocyanate)-
Supporting Information
5F10;NanoTools/Axorra)for1hatroomtemperature.Secondary antibody (OmniMap anti-Ms HRP; Ventana) was applied for 12 ... LC3 grown in complete media with or without imatinib after transfection with LAMP2 siRNA. (B) Quantification of GFP puncta per cell in GIST-T1 cells with or
DECLINEINMITOCHONDRIALFUNCTIONAFTERAUTOPHAGICSTRESSINAHUMAN ...
AnbodiesusedweretheMitoProfileTotalOXPHOSkit(Mitosciences/Abcam),LC3(NanoTools)andacn(Sigma).AnalysiswasdonebynormalizingintegratedintensiestoacnlevelsusingtheOdysseysoware.StascalanalysiswasdoneusingGraphPad(Prism). Figure2.Treatmentwith25nMBAFfor24hours inducedanaccumulaonofautophagosomes
SARS-CoV replication is severely impa ired by MG132 due to ...
148 obtained from nanoTools Antikörpertechnik GmbH & Co., KG (Teningen, Germany) and the ... 872 (A ) LC3-II signal in VeroE6 cells upon MG132 treatment. VeroE6 cells were treated with 873 DMSO, 2.5 µM MG132 or 10 µM Glivec for 16 hours.
A Combined Preclinical Therapy of Cannabinoids and ...
clonal anti–LC3 antibody (1:100; Nanotools Antik€orper-technik GmbH & Co Antik€orpertechnik GmbH & Co; clone 5F10) as primary antibodies. Incubation with Alexa-488–conjugated secondary antibody (Invitrogen) was done in the dark at room temperature for 1 hour.
Aspirin Inhibits mTOR Signaling, Activates AMP-Activated ...
LC-3 0231-100/LC3-5F10 Nanotools Reagent Aspirin A5376 Sigma Sodium salicylate S3007 Sigma Metformin D150959 Sigma Phenformin P7045 Sigma AICAR 9944 Cell Signalling [ -32P]ATP Amersham Biosciences Protein G-sepharose Amersham Biosciences
IMPLICACIÓN DE LOS FILAMENTOS DE ACTINA EN LA ARQUITECTURA ...
El anticuerpo frente LC3 (clone 5F10) es de Nanotools (Teningen, Germany), el anticuerpo frente al TGN46 humano de Serotec (Oxford, UK) y el anticuerpo ATP6J frente a la subunidad G de la H+-ATPasa vacuolar de Abcam (Cambridge, UK). Los anticuerpos